午夜精品久久久久久久久久蜜桃,亚洲精品少妇,亚洲爱爱网,国内精品人妻无码久久久影院导航

產(chǎn)品分類 / products
相關(guān)文章 / article
首頁 >> 最新促銷 >> 23227-PIERCE BCA ASSAY KIT 現(xiàn)貨Invitrogen

23227-PIERCE BCA ASSAY KIT 現(xiàn)貨Invitrogen

  • 供應(yīng)數(shù)量:757
  • 原 產(chǎn) 地:
  • 發(fā)布日期:2025/1/22
  • 已獲點(diǎn)擊:757
詳細(xì)資料

PIERCE BCA ASSAY KIT 現(xiàn)貨Invitrogen

  / 4000-520-616/

 

PIERCE BCA ASSAY KIT 現(xiàn)貨Invitrogen

 

 

 

描述

The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard.

Features of the BCA Protein Assay Kit:

• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
• Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
• Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
• Moderay fast—much easier and four times faster than the classical Lowry method
• High linearity—linear working range for BSA equals 20 to 2000 µg/mL
• Sensitive—detect down to 5 µg/mL with the enhanced protocol

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Applications:
• Studying protein:protein interactions
• Measuring column fractions after affinity chromatography
• Estimating percent recovery of membrane proteins from cell extracts
• High-throughput screening of fusion proteins

How the BCA Protein Assay Detects Protein:
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, bicinchoninic acid (BCA) reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water-soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The BCA reagent is approximay 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction that leads to BCA color formation is strongly influenced by four amino acid residues (cysteine or cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

 

 

 

如果你對(duì)23227-PIERCE BCA ASSAY KIT 現(xiàn)貨Invitrogen感興趣,想了解更詳細(xì)的產(chǎn)品信息,填寫下表直接與廠家聯(lián)系:

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
版權(quán)所有 © 2025 蘇州螞蟻淘生物科技有限公司  ICP備案號(hào):蘇ICP備17049038號(hào)-2
在線留言 聯(lián)系方式 二維碼

服務(wù)熱線

18915418616

掃一掃,關(guān)注我們

日韩亚洲欧美精品综合| 97人妻天天爽夜夜爽二区| 999国内少妇毛片视频| 欧美午夜精品久久久| 欧美第一色| a级a做爰片全部| 丁香月| 亚洲国产高清在线一区二区三区| 亚洲日韩电影欧美色图叼中文字幕| 无码国产乱人伦偷精品视频| 粗大的内捧猛烈进出在线视频| 国产精品午夜福利在线观看地址| 日本av在线| 日产精品一线二线三线芒果| 国产精品成人| 久久国产精品一区| 人成午夜免费视频在线观看| 在线观看亚洲av每日更新| 97久久超碰国产精品最新| 中文字幕亚洲综合久久菠萝蜜| 亚洲AV色香蕉一区二区蜜桃| 精品综合久久久久久888| 芒康县| 浙江省| 亚洲欧洲自拍拍偷精品网| 精品无码人妻被多人侵犯av| 国产综合久久亚洲综合 | 国产成人精品无码专区| 国产欧美久久一区二区| 高清国产AV一区二区三区| 星空无限传媒免费看电视剧 | 那曲县| 天堂av无码av在线a√| 99无码人妻一区二区三区免费| 亚洲综合色婷婷六月丁香宅男大增 | 天干天干天夜夜爽啪av| 午夜亚洲精品| 国内毛片毛片毛片| 亚洲精品一区久久久久久| 景宁| 亚洲成人一区|